> Anti-V5 Tag (V5.E10)
EBI's Anti-V5 Tag (V5.E10) mAb recognizes V5-tagged fusion proteins.
T004

Data Sheet

Print Version Anti-V5 Tag PDF Data Sheet (requires PDF Reader)
Description Anti-V5 Tag (V5.E10) Monoclonal Antibody
Clonality V5.E10
Host Species Mouse
Isotype IgG1
Immunogen GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH.
Specificity Recognizes V5-tagged fusion proteins.
Purification Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor.
Applications Dot, ELISA, IP, IS, WB (see notations).
Sensitivity WB (with ECL): 1-2ng of V5-tagged fusion proteins.
Form Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3.
Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature).
IS: 1:500-2000 dilution.
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
See Storage for more information.
Images Anti-v5 WB 01
Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).