> Anti-HA Tag (HA.C5)
EBI's Anti-HA Tag (HA.C5) mAb, similar to our Anti-His Tag (HIS.H8) mAb, has also enjoyed strong praise from our customers, due to its high affinity and high specificity.
T002

Data Sheet

Print Version Anti-HA Tag PDF Data Sheet (requires PDF Reader)
Description Anti-HA Tag (HA.C5) Monoclonal Antibody
Clonality HA.C5
Host Species Mouse
Isotype IgG3
Immunogen YPYDVPDYA (HA) synthetic peptide conjugated to KLH.
Specificity Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins.
Purification Protein A affinity chromatography from mouse ascites fluid.
Applications Dot, ELISA, IP, IS, WB (see notations).
Sensitivity WB (with ECL): 1-5ng of HA-tagged fusion proteins.
Form Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3.
Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature).
IS: 1:500-2000 dilution.
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
See Storage for more information.
Images Anti-HA WB 01
Fig. 1:1,000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2).