> Anti-ERK1/2 (E31R)
EBI's Anti-ERK1/2 (E31R) Signal Transduction mAb recognizes both the ERK1 and ERK2 proteins from human cells. ERK1 and ERK2 proteins from other species may also be detectable.
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Data Sheet

Print Version Anti-ERK1/2 PDF Data Sheet (requires PDF Reader)
Description Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody
Clonality E31R
Host Species Mouse
Isotype IgG1
Immunogen Full length recombinant human ERK1 protein.
Specificity Recognizes human ERK1 and ERK2 proteins.
Cross Reactivity WB: ERK1 and ERK2 from human cells.
ERK1 and ERK2 from other species may also be detectable.
Purification Protein A affinity chromatography from mouse ascites fluid.
Applications WB (see notations).
Form Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3.
Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions WB (with ECL): 1:1000 dilution (incubate for one hour at room temperature).
For best results with other assays, please determine optimal working dilution by titration test.
Storage Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
See Storage for more information.
Images Anti-ERK1/2-WB 01
Fig. 1:1000 (1μg/mL) Ab dilution used in WB of 10μg (1) and 30μg (2) HeLa cell lysates.