> Anti-DYKDDDDK (FLAG) Tag (FG4R)
EBI's Anti-DYKDDDDK (FLAG) Tag (FG4R) mAb recognizes N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
T006

Data Sheet

Print Version Anti-DYKDDDDK Tag PDF Data Sheet (requires PDF Reader)
Description Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody
Clonality FG4R
Host Species Mouse
Isotype IgG1
Immunogen DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.
Specificity Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
Purification Protein A affinity chromatography from mouse ascites fluid.
Applications Dot, ELISA, IP, IS, WB (see notations).
Sensitivity WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins.
Form Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3.
Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature).
IS: 1:500-2000 dilution.
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
See Storage for more information.
Images Anti-DYKDDDDK WB 01
Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2).